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验证数据展示

  • WB analysis using RMX00008

    WB analysis using RMX00008

    Untreated and DENSPM treated HEK-293 cells were were subjected to SDS PAGE followed by western blot with RMX00008 (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using RMX00008

    IHC staining of mouse brain using RMX00008

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00008 (SAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using RMX00008

    IF Staining of RAW 264.7 using RMX00008

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using SAT1 antibody (RMX00008) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb™ SAT1 Multi-Recombinant antibody

SAT1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00008

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SSAT 1, SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00008 targets SAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 spermidine/spermine N1-acetyltransferase 1
别名 SSAT 1, SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1
计算分子量 20 kDa
观测分子量 15-25 kDa
GenBank蛋白编号BC008424
基因名称 SAT1
Gene ID (NCBI) 6303
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
UNIPROT IDP21673
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAT1 antibody RMX00008Download protocol
IHC protocol for SAT1 antibody RMX00008Download protocol
IF protocol for SAT1 antibody RMX00008Download protocol
Standard Protocols
Click here to view our Standard Protocols