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验证数据展示

  • WB analysis of HEK-293 using 15162-1-AP

    WB analysis of HEK-293 using 15162-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15162-1-AP (SARS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15162-1-AP

    IHC staining of human lung cancer using 15162-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SARS antibody included
  • IHC staining of human lung cancer using 15162-1-AP

    IHC staining of human lung cancer using 15162-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 15162-1-AP (SARS antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SARS antibody included
  • IF Staining of HepG2 using 15162-1-AP

    IF Staining of HepG2 using 15162-1-AP

    Immunofluorescent analysis of HepG2 cells using 15162-1-AP (SARS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SARS Polyclonal antibody

SARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15162-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Seryl-tRNA(Ser/Sec) synthetase, Seryl-tRNA synthetase, SERS, SERRS, Serine--tRNA ligase, cytoplasmic

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SARS Ready-To-Use IHC Kit. SARS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15162-1-AP targets SARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag7270

Product name: Recombinant human SARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-514 aa of BC000716

Sequence: KKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQYALRTLGSRGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEKSDDNSYDEKYLIATSEQPIAALHRDEWLRPEDLPIKYAGLSTCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKSWEMFEEMITTAEEFYQSLGIPYHIVNIVSGSLNHAASKKLDLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMMDKVEFVHMLNATMCATTRTICAILENYQTEKGITVPEKLKEFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEGSKKKAAARDVTLENRLQNMEVTDA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 seryl-tRNA synthetase
别名 Seryl-tRNA(Ser/Sec) synthetase, Seryl-tRNA synthetase, SERS, SERRS, Serine--tRNA ligase, cytoplasmic
计算分子量 58 kDa
观测分子量 58-64 kDa
GenBank蛋白编号BC000716
基因名称 SARS
Gene ID (NCBI) 6301
RRIDAB_2184009
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49591
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SARS(Serine--tRNA ligase, cytoplasmic) Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).The deduced 514-amino acid protein has a calculated molecular mass of about 59 kD.And it can form a dimer of 120KDa(PMID:2180700).It is detected a band of 65KDa in MEL cell lines(PMID:1918033).

实验方案

Product Specific Protocols
IF protocol for SARS antibody 15162-1-APDownload protocol
IHC protocol for SARS antibody 15162-1-APDownload protocol
WB protocol for SARS antibody 15162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
View Article
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