验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
22762-1-AP targets SARDH in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SARDH fusion protein Ag18738 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sarcosine dehydrogenase |
| 别名 | BPR 2, DMGDHL1, SAR, sarcosine dehydrogenase, SARD, SARDH, SDH |
| 计算分子量 | 918 aa, 101 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC136364 |
| 基因名称 | SARDH |
| Gene ID (NCBI) | 1757 |
| RRID | AB_2879162 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL12 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SARDH antibody 22762-1-AP | Download protocol |
| IHC protocol for SARDH antibody 22762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biochem Biophys Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway | ||
Sci Rep Mutation in Smek2 regulating hepatic glucose metabolism causes hypersarcosinemia and hyperhomocysteinemia in rats | ||
Brain Res Brain transcriptomic, metabolic and mitohormesis properties associated with N-propargylglycine treatment: a prevention strategy against neurodegeneration | ||
J Cancer Sarcosine dehydrogenase as an immune infiltration-associated biomarker for the prognosis of hepatocellular carcinoma |
