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验证数据展示

  • WB analysis of HEK-293 using 12130-1-AP (same clone as 12130-1-PBS)

    WB analysis of HEK-293 using 12130-1-AP (same clone as 12130-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12130-1-AP (SAP130 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12130-1-AP (same clone as 12130-1-PBS)

    IHC staining of human pancreas cancer using 12130-1-AP (same clone as 12130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12130-1-AP (SAP130 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    IP experiment of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    IP result of anti-SAP130 (IP:12130-1-AP, 4ug; Detection:12130-1-AP 1:600) with HepG2 cells lysate 1000ug. This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    IF Staining of HepG2 using 12130-1-AP (same clone as 12130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAP130 antibody (12130-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 12130-1-PBS in a different storage buffer formulation.

SAP130 Polyclonal antibody, PBS Only

SAP130 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12130-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Sin3-associated polypeptide p130, 130 kDa Sin3-associated polypeptide

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12130-1-PBS targets SAP130 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2774

Product name: Recombinant human SAP130 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 354-693 aa of BC017453

Sequence: PTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQHVISTEEGDMMETNSTDDEKSTAKSLLVKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLLRHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTNLEHDVYERLTNLQEGIIPKKKAATDDDLHRINELIQGNMQRCKLVMDQISEARDSMLKVLDHKDRVLKLLNKNGTVKKVSKLKRKEKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sin3A-associated protein, 130kDa
别名 Sin3-associated polypeptide p130, 130 kDa Sin3-associated polypeptide
计算分子量 110 kDa
观测分子量 130 kDa
GenBank蛋白编号BC017453
基因名称 SAP130
Gene ID (NCBI) 79595
RRIDAB_2186637
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0E3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

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