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- KD/KO Validated
CoraLite® Plus 488-conjugated SAMHD1 Polyclonal antibody
SAMHD1 Polyclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 12586-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:300-1:1200 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12586 targets SAMHD1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | human, mouse, rat |
| Immunogen | SAMHD1 fusion protein Ag3287 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SAM domain and HD domain 1 |
| Synonyms | DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88 |
| Calculated Molecular Weight | 626 aa, 72 kDa |
| Observed Molecular Weight | 64-72 kDa |
| GenBank Accession Number | BC036450 |
| Gene Symbol | SAMHD1 |
| Gene ID (NCBI) | 25939 |
| RRID | AB_2919057 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3Z3 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with a presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SAMHD1 antibody CL488-12586 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
