验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human spleen tissue, human kidney tissue, human lung tissue, human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
17387-1-AP targets SAMD4A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SAMD4A fusion protein Ag11273 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sterile alpha motif domain containing 4A |
| 别名 | hSmaug1, KIAA1053, Protein Smaug homolog 1, SAMD4, SAMD4A, Smaug, Smaug 1, Smaug1, SMG, SMGA |
| 计算分子量 | 529 aa, 59 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC021726 |
| 基因名称 | SAMD4A |
| Gene ID (NCBI) | 23034 |
| RRID | AB_2301339 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UPU9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAMD4A antibody 17387-1-AP | Download protocol |
| IHC protocol for SAMD4A antibody 17387-1-AP | Download protocol |
| IP protocol for SAMD4A antibody 17387-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.
| ||
J Neuroinflammation Smad7 in the hippocampus contributes to memory impairment in aged mice after anesthesia and surgery | ||
Tissue Cell SAMD4A serves as a negative prognostic marker for gastric cancer patients
| ||
Cell Host Microbe MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids | ||
Sci Rep GNG2 inhibits brain metastases from colorectal cancer via PI3K/AKT/mTOR signaling pathway |
