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验证数据展示

  • WB analysis of Jurkat using 23114-1-AP

    WB analysis of Jurkat using 23114-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23114-1-AP (SALL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SALL2 Polyclonal antibody

SALL2 Polyclonal Antibody for WB, ELISA
Cat No. 23114-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HSAL2, KIAA0360, p150(Sal2), Sal 2, sal like 2 (Drosophila), Sal like protein 2, SAL2, SALL2, Zinc finger protein SALL2, Zinc finger protein Spalt 2, ZNF795

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23114-1-AP targets SALL2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19427

Product name: Recombinant human SALL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 753-1007 aa of BC136528

Sequence: SQQPSPEEELSEEEEEEDEEEEEDVTDEDSLAGRGSESGGEKAISVRGDSEEASGAEEEVGTVAAAATAGKEMDSNEKTTQQSSLPPPPPPDSLDQPQPMEQGSSGVLGGKEEGGKPERSSSPASALTPEGEATSVTLVEELSLQEAMRKEPGESSSRKACEVCGQAFPSQAALEEHQKTHPKEGPLFTCVFCRQGFLERATLKKHMLLAHHQVQPFAPHGPQNIAALSLVPGCSPSITSTGLSPFPRKDDPTIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sal-like 2 (Drosophila)
别名 HSAL2, KIAA0360, p150(Sal2), Sal 2, sal like 2 (Drosophila), Sal like protein 2, SAL2, SALL2, Zinc finger protein SALL2, Zinc finger protein Spalt 2, ZNF795
计算分子量 1007 aa, 105 kDa
观测分子量 130 kDa
GenBank蛋白编号BC136528
基因名称 SALL2
Gene ID (NCBI) 6297
RRIDAB_2879212
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9Y467
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sall2 (SALL2, sal-like 2, HSAL2, p150(Sal2)) are mammalian homologs of the Drosophila region-specific home-otic gene spalt (sal), which encodes a zinc finger-containing transcription regulator. Mammalian Sall1 may mediate higher order chromatin structure and may be a component of a distinct heterochromatin-dependent silencing process. Sall2 is a p53-independent regulator of p21 and BAX, and can function in some cell types as a regulator of cell growth and survival. Human Sall2 is present in brain, heart, kidney and pancreas. This antibody is specific to the SALL2 protein.

实验方案

Product Specific Protocols
WB protocol for SALL2 antibody 23114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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