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验证数据展示

  • IHC staining of human skin cancer using 30914-1-AP (same clone as 30914-1-PBS)

    IHC staining of human skin cancer using 30914-1-AP (same clone as 30914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 30914-1-AP (same clone as 30914-1-PBS)

    IF Staining of A375 using 30914-1-AP (same clone as 30914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30914-1-AP (same clone as 30914-1-PBS)

    IF Staining of U2OS using 30914-1-AP (same clone as 30914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

SAGE1 Polyclonal antibody, PBS Only

SAGE1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 30914-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30914-1-PBS targets SAGE1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34216

Product name: Recombinant human SAGE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 247-387 aa of NM_018666.2

Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sarcoma antigen 1
别名 Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1
计算分子量99kd
GenBank蛋白编号NM_018666.2
基因名称 SAGE1
Gene ID (NCBI) 55511
RRIDAB_3669783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NXZ1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596). It is primarily found in primates and is generally absent in lower mammals like rodents

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