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验证数据展示

  • IHC staining of human skin cancer using 30914-1-AP

    IHC staining of human skin cancer using 30914-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 30914-1-AP

    IHC staining of rat testis using 30914-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30914-1-AP

    IHC staining of mouse testis using 30914-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A375 using 30914-1-AP

    IF Staining of A375 using 30914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of U2OS using 30914-1-AP

    IF Staining of U2OS using 30914-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SAGE1 Polyclonal antibody

SAGE1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30914-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman skin cancer tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30914-1-AP targets SAGE1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34216

Product name: Recombinant human SAGE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 247-387 aa of NM_018666.2

Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sarcoma antigen 1
别名 sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14
计算分子量99kd
GenBank蛋白编号NM_018666.2
基因名称 SAGE1
Gene ID (NCBI) 55511
RRIDAB_3669783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NXZ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).

实验方案

Product Specific Protocols
IF protocol for SAGE1 antibody 30914-1-APDownload protocol
IHC protocol for SAGE1 antibody 30914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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