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验证数据展示

  • IHC staining of human colon cancer using 84516-6-RR

    IHC staining of human colon cancer using 84516-6-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84516-6-RR (SAE1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 84516-6-RR

    IHC staining of human colon cancer using 84516-6-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84516-6-RR (SAE1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84516-6-RR

    IF Staining of HeLa using 84516-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SAE1 antibody (84516-6-RR, Clone: 241923F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of MCF-7 using 84516-6-RR

    IF Staining of MCF-7 using 84516-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SAE1 antibody (84516-6-RR, Clone: 241923F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A549 using 84516-6-RR

    FC experiment of A549 using 84516-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SAE1 Recombinant antibody (84516-6-RR, Clone:241923F12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84516-6-RR

    Affinity and Kinetic Characterization of 84516-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84516-6-RR against Human SAE1 were performed. The affinity constant is 1.52 nM.

SAE1 Recombinant monoclonal antibody

SAE1 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84516-6-RR

产品说明书

CloneNo. 241923F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

241923F12, AOS1, HSPC140, Sentrin/SUMO activating protein AOS1, SUA1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84516-6-RR targets SAE1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0275

Product name: Recombinant human SAE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-207 aa of BC000344

Sequence: MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1 activating enzyme subunit 1
别名 241923F12, AOS1, HSPC140, Sentrin/SUMO activating protein AOS1, SUA1
计算分子量 38 kDa
GenBank蛋白编号BC000344
基因名称 SAE1
Gene ID (NCBI) 10055
RRIDAB_3672022
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBE0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

实验方案

Product Specific Protocols
FC protocol for SAE1 antibody 84516-6-RRDownload protocol
IF protocol for SAE1 antibody 84516-6-RRDownload protocol
IHC protocol for SAE1 antibody 84516-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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