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SAC3D1 Polyclonal antibody

SAC3D1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25857-1-AP

Print datasheet

Synonyms

HSU79266, SAC3 domain containing 1, SAC3D1, SHD1

验证数据展示

  • WB analysis using 25857-1-AP

    WB analysis using 25857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25857-1-AP

    IHC staining of human liver cancer using 25857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 25857-1-AP

    IHC staining of human liver cancer using 25857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 25857-1-AP

    WB analysis using 25857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, PC-3 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25857-1-AP is SAC3D1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen SAC3D1 fusion protein Ag23026 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SAC3 domain containing 1
Synonyms HSU79266, SAC3 domain containing 1, SAC3D1, SHD1
Calculated Molecular Weight 404 aa, 44 kDa
Observed Molecular Weight44, 31 kDa
GenBank Accession NumberBC007448
Gene Symbol SAC3D1
Gene ID (NCBI) 29901
RRIDAB_2918096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDF8WC89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAC3D1, also named as SHD1 and HSU79266. SAC3D1 is widely found in the cytoplasm, cytoskeleton, microtubule, tissue center, centrosome and spindle, involved in centrosome duplication and mitotic progression. SAC3D1 is abnormally expressed in multiple types of cancer, such as colon cancer and hepatocellular carcinoma and gastric cancer. SAC3D1 may serve as a prognostic biomarker in hepatocellular carcinoma (PMID: 30353105, 32319600). SAC3D1 has 2 isoforms with the molecular mass of 44 and 31 kDa.

实验方案

Product Specific Protocols
WB protocol for SAC3D1 antibody 25857-1-APDownload protocol
IHC protocol for SAC3D1 antibody 25857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols