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验证数据展示

  • WB analysis using 25857-1-AP

    WB analysis using 25857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25857-1-AP

    IHC staining of human liver cancer using 25857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 25857-1-AP

    IHC staining of human liver cancer using 25857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 25857-1-AP

    WB analysis using 25857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25857-1-AP

    IHC staining of human colon cancer using 25857-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SAC3D1 Polyclonal antibody

SAC3D1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25857-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

SHD1, SAC3 domain containing 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, PC-3 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25857-1-AP targets SAC3D1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 SAC3D1 fusion protein Ag23026 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SAC3 domain containing 1
别名 SHD1, SAC3 domain containing 1
计算分子量 404 aa, 44 kDa
观测分子量44, 31 kDa
GenBank蛋白编号BC007448
基因名称 SAC3D1
Gene ID (NCBI) 29901
RRIDAB_2918096
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDF8WC89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAC3D1, also named as SHD1 and HSU79266. SAC3D1 is widely found in the cytoplasm, cytoskeleton, microtubule, tissue center, centrosome and spindle, involved in centrosome duplication and mitotic progression. SAC3D1 is abnormally expressed in multiple types of cancer, such as colon cancer and hepatocellular carcinoma and gastric cancer. SAC3D1 may serve as a prognostic biomarker in hepatocellular carcinoma (PMID: 30353105, 32319600). SAC3D1 has 2 isoforms with the molecular mass of 44 and 31 kDa.

实验方案

Product Specific Protocols
WB protocol for SAC3D1 antibody 25857-1-APDownload protocol
IHC protocol for SAC3D1 antibody 25857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Front Genet

Conduction and validation of a novel mitotic spindle assembly related signature in hepatocellular carcinoma: prognostic prediction, tumor immune microenvironment and drug susceptibility

Authors - Zhao Zhang
  • KD Validated
View Article