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SAAL1 Polyclonal antibody

SAAL1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25467-1-AP

Print datasheet

Synonyms

Protein SAAL1, SAAL1, serum amyloid A like 1

验证数据展示

  • WB analysis of HepG2 using 25467-1-AP

    WB analysis of HepG2 using 25467-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25467-1-AP (SAAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25467-1-AP

    IF Staining of HepG2 using 25467-1-AP

    Immunofluorescent analysis of HepG2 cells using 25467-1-AP (SAAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 25467-1-AP is SAAL1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SAAL1 fusion protein Ag22186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serum amyloid A-like 1
Synonyms Protein SAAL1, SAAL1, serum amyloid A like 1
Calculated Molecular Weight 475 aa, 54 kDa
Observed Molecular Weight 54-55 kDa
GenBank Accession NumberBC012010
Gene Symbol SAAL1
Gene ID (NCBI) 113174
RRIDAB_2880094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96ER3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAAL1 antibody 25467-1-APDownload protocol
IF protocol for SAAL1 antibody 25467-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

BMC Cancer

The effects of the prognostic biomarker SAAL1 on cancer growth and its association with the immune microenvironment in lung adenocarcinoma

Authors - Song Tong
View Article
mouseWB

Front Pharmacol

Bicyclol attenuates high fat diet-induced non-alcoholic fatty liver disease/non-alcoholic steatohepatitis through modulating multiple pathways in mice

Authors - Jingyi Wu
View Article