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验证数据展示

  • WB analysis using 88256-7-RR

    WB analysis using 88256-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88256-7-RR (S100P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 88256-7-RR

    IHC staining of human placenta using 88256-7-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88256-7-RR (S100P antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100P antibody included

S100P Recombinant monoclonal antibody

S100P Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 88256-7-RR

产品说明书

CloneNo. 260786F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Migration-inducing gene 9 protein, Protein S100 P, Protein S100-E, Protein S100-P, S100 calcium binding protein P

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HT-29 cells, COLO 205 cells, HuH-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy S100P Ready-To-Use IHC Kit. S100P primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88256-7-RR targets S100P in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2379

Product name: Recombinant human S100P protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-95 aa of BC006819

Sequence: MTELETAMGMIIDVFSRYSGSEGSTQTLTKGELKVLMEKELPGFLQSGKDKDAVDKLLKDLDANGDAQVDFSEFIVFVAAITSACHKYFEKAGLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein P
别名 Migration-inducing gene 9 protein, Protein S100 P, Protein S100-E, Protein S100-P, S100 calcium binding protein P
计算分子量 95 aa, 11 kDa
观测分子量11 kDa
GenBank蛋白编号BC006819
基因名称 S100P
Gene ID (NCBI) 6286
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25815
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

实验方案

Product Specific Protocols
IHC protocol for S100P antibody 88256-7-RRDownload protocol
WB protocol for S100P antibody 88256-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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