Various lysates were subjected to SDS PAGE followed by western blot with 88256-7-RR (S100P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88256-7-PBS in a different storage buffer formulation.

The mean S100P concentration was determined to be 28.55 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 99.89 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load, 9.63 ng/mL in Ramos cell extract based on a 1.20 mg/mL extract load, 13.25 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load and 12.60 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load.

Serum of eight individual healthy human donors was measured. The S100P concentration of detected samples was determined to be 1.74 ng/mL with a range of 0.51-4.21 ng/mL.

Sandwich ELISA standard curve of MP03518-2, Human S100P Recombinant Matched Antibody Pair - PBS only. 88256-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2379. 88256-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 88256-7-RR (S100P antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88256-7-PBS in a different storage buffer formulation.