S100P Polyclonal antibody
S100P Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HuH-7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human colon cancer tissue, human lung cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy S100P Ready-To-Use IHC Kit. S100P primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 6 publications below |
产品信息
11803-1-AP targets S100P in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | S100P fusion protein Ag2379 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | S100 calcium binding protein P |
| Synonyms | MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P |
| Calculated Molecular Weight | 95 aa, 11 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC006819 |
| Gene Symbol | S100P |
| Gene ID (NCBI) | 6286 |
| RRID | AB_2184577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25815 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S100P antibody 11803-1-AP | Download protocol |
| IHC protocol for S100P antibody 11803-1-AP | Download protocol |
| IF protocol for S100P antibody 11803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation | ||
Am J Cancer Res High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer. | ||
Proteomics Clin Appl Identification of distinctive protein expression patterns in colorectal adenoma. | ||
Diagn Pathol Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion. | ||
Exp Ther Med Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma. | ||
Technol Cancer Res Treat LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p |
