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验证数据展示

  • WB analysis of MCF-7 using 83578-2-RR (same clone as 83578-2-PBS)

    WB analysis of MCF-7 using 83578-2-RR (same clone as 83578-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • WB analysis using 83578-2-PBS

    WB analysis using 83578-2-PBS

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-PBS (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83578-2-RR (same clone as 83578-2-PBS)

    WB analysis using 83578-2-RR (same clone as 83578-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83578-2-RR (same clone as 83578-2-PBS)

    IF Staining of A549 using 83578-2-RR (same clone as 83578-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83578-2-RR (same clone as 83578-2-PBS)

    IF Staining of HeLa using 83578-2-RR (same clone as 83578-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83578-2-PBS

    Affinity and Kinetic Characterization of 83578-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83578-2-PBS against Human S100A9 was performed. The affinity constant is 0.389 nM.

  • Affinity and Kinetic Characterization of 83578-2-RR

    Affinity and Kinetic Characterization of 83578-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

S100A9 Recombinant antibody, PBS Only

S100A9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83578-2-PBS

产品说明书

CloneNo. 240539G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

240539G9, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, MCF-7 cells, HL-60 cells
Planning an IHC experiment? We recommend our IHCeasy S100A9 Ready-To-Use IHC Kit. S100A9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83578-2-PBS targets S100A9 in WB, IF/ICC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 S100A9 fusion protein Ag25764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A9
别名 240539G9, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit
计算分子量 13 kDa
观测分子量 14 kDa
GenBank蛋白编号BC047681
基因名称 S100A9
Gene ID (NCBI) 6280
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06702
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

实验方案

Product Specific Protocols
WB protocol for S100A9 antibody 83578-2-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols