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验证数据展示

  • FC experiment of Transfected HEK-293T using CL594-98467-2

    FC experiment of Transfected HEK-293T using CL594-98467-2

    1x10^6 HEK-293T cells (left) or S100A8-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse S100A8 Rabbit RecAb (CL594-98467-2, Clone: 250113D8). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • FC experiment of Transfected HEK-293T using CL594-98467-2

    FC experiment of Transfected HEK-293T using CL594-98467-2

    1x10^6 HEK-293T cells (left) or S100A8-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse S100A8 Rabbit RecAb (CL594-98467-2, Clone: 250113D8) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

CoraLite®594 Anti-Mouse S100A8 Rabbit Recombinant Antibody

S100A8 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL594-98467-2

产品说明书

CloneNo. 250113D8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 98467-2-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-98467-2 targets S100A8 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2959

Product name: Recombinant Mouse S100A8 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 2-89 aa of NM_013650.2

Sequence: PSELEKALSNLIDVYHNYSNIQGNHHALYKNDFKKMVTTECPQFVQNINIENLFRELDINSDNAINFEEFLAMVIKVGVASHKDSHKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A8 (calgranulin A)
别名 Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8
计算分子量10 kDa
GenBank蛋白编号NM_013650.2
基因名称 S100a8
Gene ID (NCBI) 20201
RRIDAB_3748457
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
激发激光Yellow-Green Laser (561 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP27005
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

实验方案

Product Specific Protocols
FC protocol for CL594 S100A8 antibody CL594-98467-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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