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验证数据展示

  • IF Staining of human tonsillitis using CL488-66853

    IF Staining of human tonsillitis using CL488-66853

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 S100A8 antibody (CL488-66853, Clone: 1G11C8 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL488-66853

    IF Staining of human tonsillitis using CL488-66853

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 S100A8 antibody (CL488-66853, Clone: 1G11C8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated S100A8 Monoclonal antibody

S100A8 Monoclonal Antibody for IF-P
Cat No. CL488-66853

产品说明书

CloneNo. 1G11C8

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF-P

别名

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy S100A8 Ready-To-Use IHC Kit. S100A8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66853-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66853 targets S100A8 in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原

CatNo: Ag8517

Product name: Recombinant human S100A8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-93 aa of BC005928

Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 S100 calcium binding protein A8
别名 60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A
计算分子量 93 aa, 11 kDa
GenBank蛋白编号BC005928
基因名称 S100A8
Gene ID (NCBI) 6279
RRIDAB_2919379
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05109
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 S100A8 antibody CL488-66853Download protocol
Standard Protocols
Click here to view our Standard Protocols
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