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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using 98411-3-RR

    FC experiment of human peripheral blood lymphocytes using 98411-3-RR

    1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4),or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human peripheral blood lymphocytes using 98411-3-RR

    FC experiment of human peripheral blood lymphocytes using 98411-3-RR

    1x10^6 human peripheral blood lymphocytes were intracellularly stained with 0.25 ug Anti-Human S100A8 Rabbit RecAb (98411-3-RR, Clone: 242970C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Anti-Human S100A8 Rabbit Recombinant Antibody

S100A8 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98411-3-RR

产品说明书

CloneNo. 242970C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy S100A8 Ready-To-Use IHC Kit. S100A8 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98411-3-RR targets S100A8 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Eg3752

Product name: Recombinant Human S100A8 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 1-93 aa of BC005928

Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A8
别名 CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG
计算分子量 93 aa, 11 kDa
GenBank蛋白编号BC005928
基因名称 S100A8
Gene ID (NCBI) 6279
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05109
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

实验方案

Product Specific Protocols
FC protocol for S100A8 antibody 98411-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols