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验证数据展示

  • WB analysis using 82531-3-RR (same clone as 82531-3-PBS)

    WB analysis using 82531-3-RR (same clone as 82531-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82531-3-RR (S100A8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82531-3-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82531-3-RR (same clone as 82531-3-PBS)

    IP experiment of MCF-7 using 82531-3-RR (same clone as 82531-3-PBS)

    IP result of anti-S100A8 (IP:82531-3-RR, 4ug; Detection:82531-3-RR 1:600) with MCF-7 cells lysate 1400 ug. This data was developed using the same antibody clone with 82531-3-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 82531-3-RR (same clone as 82531-3-PBS)

    IP experiment of HL-60 using 82531-3-RR (same clone as 82531-3-PBS)

    IP result of anti-S100A8 (IP:82531-3-RR, 4ug; Detection:82531-3-RR 1:600) with HL-60 cells lysate 1800 ug. This data was developed using the same antibody clone with 82531-3-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 82531-3-RR (same clone as 82531-3-PBS)

    IF Staining of THP-1 using 82531-3-RR (same clone as 82531-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using S100A8 antibody (82531-3-RR, Clone: 242970G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82531-3-PBS in a different storage buffer formulation.

S100A8 Recombinant monoclonal antibody, PBS Only

S100A8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82531-3-PBS

产品说明书

CloneNo. 242970G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy S100A8 Ready-To-Use IHC Kit. S100A8 primary antibody included.

产品信息

82531-3-PBS targets S100A8 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3752

Product name: Recombinant Human S100A8 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 1-93 aa of BC005928

Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A8
别名 CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG
计算分子量 93 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC005928
基因名称 S100A8
Gene ID (NCBI) 6279
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05109
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

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