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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-10237

Print datasheet

Synonyms

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C

验证数据展示

  • FC experiment of MCF-7 using CL647-10237

    FC experiment of MCF-7 using CL647-10237

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human S100A11 (CL647-10237) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A11 Ready-To-Use IHC Kit. S100A11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10237-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10237 targets S100A11 in FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, rat, mouse
Immunogen S100A11 fusion protein Ag0343 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A11
Synonyms Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C
Calculated Molecular Weight 105 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC001410
Gene Symbol S100A11
Gene ID (NCBI) 6282
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31949
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 S100A11 antibody CL647-10237Download protocol
Standard Protocols
Click here to view our Standard Protocols