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- KD/KO Validated
S100A10 Polyclonal antibody
S100A10 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
FC, IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HT-29 cells, human lung tissue, HaCaT cells |
Positive IHC detected in | human lung cancer tissue, human gliomas tissue, human pancreas cancer tissue, human skin tissue, human cervical cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11250-1-AP targets S100A10 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, FC, IHC, ELISA |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | S100A10 fusion protein Ag1779 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | S100 calcium binding protein A10 |
Synonyms | 42C, ANX2L, ANX2LG, CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10 |
Calculated Molecular Weight | 11 kDa |
Observed Molecular Weight | 11 kDa |
GenBank Accession Number | BC015973 |
Gene Symbol | S100A10 |
Gene ID (NCBI) | 6281 |
RRID | AB_2269906 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P60903 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for S100A10 antibody 11250-1-AP | Download protocol |
IHC protocol for S100A10 antibody 11250-1-AP | Download protocol |
IF protocol for S100A10 antibody 11250-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Mol Immunol Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection. | ||
Diabetes Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.
| ||
J Nanobiotechnology Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury | ||
J Med Chem 5α-Epoxyalantolactone Inhibits Metastasis of Triple-Negative Breast Cancer Cells by Covalently Binding a Conserved Cysteine of Annexin A2. | ||
Cancers (Basel) S100A10 Has a Critical Regulatory Function in Mammary Tumor Growth and Metastasis: Insights Using MMTV-PyMT Oncomice and Clinical Patient Sample Analysis.
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J Neurosci Blocking the Thrombin Receptor Promotes Repair of Demyelinated Lesions in the Adult Brain. |