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验证数据展示

  • WB analysis using 88164-1-RR

    WB analysis using 88164-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88164-1-RR (S100A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 88164-1-RR

    IHC staining of human tonsillitis using 88164-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88164-1-RR (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A1 antibody included
  • IHC staining of human tonsillitis using 88164-1-RR

    IHC staining of human tonsillitis using 88164-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88164-1-RR (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100A1 antibody included
  • IF Staining of A375 using 88164-1-RR

    IF Staining of A375 using 88164-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using S100A1 antibody (88164-1-RR, Clone: 252961A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

S100A1 Recombinant monoclonal antibody

S100A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 88164-1-RR

产品说明书

CloneNo. 252961A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Protein S100 A1, Protein S100-A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100 calcium-binding protein A1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy S100A1 Ready-To-Use IHC Kit. S100A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88164-1-RR targets S100A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8871

Product name: Recombinant human S100A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-94 aa of BC014392

Sequence: MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A1
别名 Protein S100 A1, Protein S100-A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100 calcium-binding protein A1
计算分子量 94 aa, 11 kDa
观测分子量 10 kDa
GenBank蛋白编号BC014392
基因名称 S100A1
Gene ID (NCBI) 6271
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100A1 belongs to the S100 protein family, which represents the largest group among EF-hand Ca2+-binding proteins exclusively expressed in vertebrates. S100 proteins have been described as central regulators of numerous cellular and molecular processes, often operating in a cell-type-specific manner (PMID: 11991838). S100A1 is a central regulator of Ca2+-driven networks that orchestrates compartmentalized Ca2+ fluxes in the heart (PMID: 22336719, PMID: 26021637). S100A1 is primarily expressed in cardiomyocytes (CM). Endothelial cells (EC) express significantly lower S100A1 levels, whereas fibroblasts do not express S100A1 (PMID: 38641766).

实验方案

Product Specific Protocols
IF protocol for S100A1 antibody 88164-1-RRDownload protocol
IHC protocol for S100A1 antibody 88164-1-RRDownload protocol
WB protocol for S100A1 antibody 88164-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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