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验证数据展示

  • WB analysis using 88164-1-RR (same clone as 88164-1-PBS)

    WB analysis using 88164-1-RR (same clone as 88164-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88164-1-RR (S100A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88164-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 88164-1-RR (same clone as 88164-1-PBS)

    IHC staining of human tonsillitis using 88164-1-RR (same clone as 88164-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88164-1-RR (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88164-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A1 antibody included
  • IHC staining of human tonsillitis using 88164-1-RR (same clone as 88164-1-PBS)

    IHC staining of human tonsillitis using 88164-1-RR (same clone as 88164-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88164-1-RR (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88164-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A1 antibody included
  • IF Staining of A375 using 88164-1-RR (same clone as 88164-1-PBS)

    IF Staining of A375 using 88164-1-RR (same clone as 88164-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A375 cells using S100A1 antibody (88164-1-RR, Clone: 252961A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88164-1-PBS in a different storage buffer formulation.

S100A1 Recombinant monoclonal antibody, PBS Only

S100A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 88164-1-PBS

产品说明书

CloneNo. 252961A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein S100 A1, Protein S100-A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100 calcium-binding protein A1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy S100A1 Ready-To-Use IHC Kit. S100A1 primary antibody included.

产品信息

88164-1-PBS targets S100A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8871

Product name: Recombinant human S100A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-94 aa of BC014392

Sequence: MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 S100 calcium binding protein A1
别名 Protein S100 A1, Protein S100-A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100 calcium-binding protein A1
计算分子量 94 aa, 11 kDa
观测分子量 10 kDa
GenBank蛋白编号BC014392
基因名称 S100A1
Gene ID (NCBI) 6271
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23297
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A1 belongs to the S100 protein family, which represents the largest group among EF-hand Ca2+-binding proteins exclusively expressed in vertebrates. S100 proteins have been described as central regulators of numerous cellular and molecular processes, often operating in a cell-type-specific manner (PMID: 11991838). S100A1 is a central regulator of Ca2+-driven networks that orchestrates compartmentalized Ca2+ fluxes in the heart (PMID: 22336719, PMID: 26021637). S100A1 is primarily expressed in cardiomyocytes (CM). Endothelial cells (EC) express significantly lower S100A1 levels, whereas fibroblasts do not express S100A1 (PMID: 38641766).

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