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验证数据展示

  • IF Staining of HepG2 using CL488-67028

    IF Staining of HepG2 using CL488-67028

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Ribosomal protein L4 antibody (CL488-67028, Clone: 3C7A8 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67028

    FC experiment of HepG2 using CL488-67028

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Ribosomal protein L4 (CL488-67028, Clone:3C7A8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated Ribosomal protein L4 Monoclonal antibody

Ribosomal protein L4 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67028

产品说明书

CloneNo. 3C7A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67028-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67028 targets Ribosomal protein L4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein L4
别名 RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC009888
基因名称 Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_3084300
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP36578
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Ribosomal protein L4 antibody CL488-67028Download protocol
FC protocol for CL Plus 488 Ribosomal protein L4 antibody CL488-67028Download protocol
Standard Protocols
Click here to view our Standard Protocols