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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80959-1-RR (same clone as 80959-1-PBS)

    WB analysis of HEK-293 using 80959-1-RR (same clone as 80959-1-PBS)

    WB result of Ribosomal protein L4 antibody (80959-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ribosomal protein L4 transfected HEK-293 cells. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

    IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL4 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RPL4 antibody included
  • FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Ribosomal protein L4 Recombinant antibody, PBS Only

Ribosomal protein L4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80959-1-PBS

产品说明书

CloneNo. 3K6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

RPL4, 3K6, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPL4 Ready-To-Use IHC Kit. RPL4 primary antibody included.

产品信息

80959-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Ribosomal protein L4 fusion protein Ag28728 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L4
别名 RPL4, 3K6, 60S ribosomal protein L1, 60S ribosomal protein L4, RPL 4
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC009888
基因名称 Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2918922
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36578
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.