验证数据展示
经过测试的应用
| Positive WB detected in | ag27669 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 1 publications below |
产品信息
67378-1-Ig targets eqRFP630/eqRFP611 in WB, IF, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human, mouse, yeast |
| 免疫原 |
CatNo: Ag27669 Product name: Recombinant entacmaea quadricolor Red fluorescent protein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-231 aa of Sequence: MNSLIKENMRMMVVMEGSVNGYQFKCTGEGDGNPYMGTQTMRIKVVEGGPLPFAFDILATSFMYGSKTFIKHTKGIPDFFKQSFPEGFTWERVTRYEDGGVFTVMQDTSLEDGCLVYHAKVTGVNFPSNGAVMQKKTKGWEPNTEMLYPADGGLRGYSQMALNVDGGGYLSCSFETTYRSKKTVENFKMPGFHFVDHRLERLEESDKEMFVVQHEHAVAKFCDLPSKLGRL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | eqRFP630/eqRFP611 |
| 别名 | Red fluorescent protein, eqFP611, RFP tag, Red fluorescent protein eqFP611, GFP-like chromoprotein |
| 计算分子量 | 26 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2882625 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8ISF8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. RFP611 and RFP 630 is a basic (constitutively fluorescent) red fluorescent protein derived from Entacmaea quadricolor. This antibody is a mouse (IgG2b) monoclonal antibody raised against RFP (eqFP611), it can also recognize eqFP630. This antibody does not recognize DsRed or mRFP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for eqRFP630/eqRFP611 antibody 67378-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins | ||
PNAS Nexus Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability | ||
Autophagy The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple | ||
EMBO Rep Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome | ||
Mol Biol Cell Membrane binding of endocytic myosin-1s is inhibited by a class of ankyrin repeat proteins |