Rat IL-2 ELISA Kit
Sensitivity
0.5 pg/mL
Range
7.8-500 pg/mL
Reactivity
Rat
Validation Data Gallery
Product Information
KE20019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-2. The assay recognizes rat IL-2. An antibody specific for IL-2 has been pre-coated onto the microwells. The IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for rat IL-2 is added to detect the captured rat IL-2 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 116562 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 100% | 76%-118% |
| Cell culture supernatant | 101% | 88%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 518.6 | 31.2 | 6.0 |
| 2 | 20 | 109.4 | 4.0 | 3.7 |
| 3 | 20 | 27.1 | 1.4 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 528.7 | 27.5 | 5.2 |
| 2 | 24 | 114.3 | 4.7 | 4.1 |
| 3 | 24 | 29.0 | 1.2 | 4.1 |
Background Information
IL-2 (Interleukin-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells. IL-2 mediates its effects by binding to IL-2 receptors which are expressed by lymphocytes. IL-2 signal can be transduced by 3 different signaling pathways: JAK-STAT, PI3K/Akt/mTOR and MAPK/ERK pathway. IL-2 plays a role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. It also promotes proliferation of activated B cells and subsequently immunoglobulin production. IL-2 has been used to treat immune-deficiencies like HIV, and induce immune response against tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il2, interleukin 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
