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验证数据展示

  • WB analysis using 20984-1-AP (same clone as 20984-1-PBS)

    WB analysis using 20984-1-AP (same clone as 20984-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 cells using 20984-1-AP (same clone as 20984-1-PBS)

    WB analysis of HepG2 cells using 20984-1-AP (same clone as 20984-1-PBS)

    WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 20984-1-AP (same clone as 20984-1-PBS)

    IP experiment of HEK-293 using 20984-1-AP (same clone as 20984-1-PBS)

    IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocellular carcinoma using 20984-1-AP (same clone as 20984-1-PBS)

    IHC staining of human hepatocellular carcinoma using 20984-1-AP (same clone as 20984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma slide using 20984-1-AP (Raptor antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Raptor antibody included
  • IF Staining of U2OS using 20984-1-AP (same clone as 20984-1-PBS)

    IF Staining of U2OS using 20984-1-AP (same clone as 20984-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Raptor Polyclonal antibody, PBS Only

Raptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20984-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

KIAA1303, p150 target of rapamycin, Regulatory-associated protein of mTOR, RPTOR, TOR

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Raptor Ready-To-Use IHC Kit. Raptor primary antibody included.

产品信息

20984-1-PBS targets Raptor in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 raptor
别名 KIAA1303, p150 target of rapamycin, Regulatory-associated protein of mTOR, RPTOR, TOR
计算分子量 149 kDa
观测分子量 130-150 kDa
GenBank蛋白编号NM_020761
基因名称 Raptor
Gene ID (NCBI) 57521
RRIDAB_11182390
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N122
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.

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