验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-27587 targets RYR2 in IF-P applications and shows reactivity with Human, mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 |
CatNo: Ag26296 Product name: Recombinant human RYR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1311-1410 aa of NM_001035 Sequence: MAEVFSKTVAGGLPGAGLFGPKNDLEDYDADSDFEVLMKTAHGHLVPDRVDKDKEATKPEFNNHKDYAQEKPSRLKQRFLLRRTKPDYSTSHSARLTEDVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ryanodine receptor 2 (cardiac) |
| 别名 | ARVC2, ARVD2, ryanodine receptor 2 (cardiac), RYR2, VTSIP |
| 计算分子量 | 565 kDa |
| GenBank蛋白编号 | NM_001035 |
| 基因名称 | RYR2 |
| Gene ID (NCBI) | 6262 |
| RRID | AB_2923952 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92736 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 RYR2 antibody CL647-27587 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
