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验证数据展示

  • WB analysis using 67851-1-Ig (same clone as 67851-1-PBS)

    WB analysis using 67851-1-Ig (same clone as 67851-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67851-1-Ig (RUVBL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67851-1-Ig (same clone as 67851-1-PBS)

    IHC staining of human lung cancer using 67851-1-Ig (same clone as 67851-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RUVBL2 antibody included
  • IF Staining of HeLa using 67851-1-Ig (same clone as 67851-1-PBS)

    IF Staining of HeLa using 67851-1-Ig (same clone as 67851-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RUVBL2 antibody (67851-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

RUVBL2 Monoclonal antibody, PBS Only

RUVBL2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67851-1-PBS

产品说明书

CloneNo. 1A1A4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RUVBL2 Ready-To-Use IHC Kit. RUVBL2 primary antibody included.

产品信息

67851-1-PBS targets RUVBL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 RUVBL2 fusion protein Ag0253 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 RuvB-like 2 (E. coli)
别名 48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B
计算分子量 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC000428
基因名称 RUVBL2
Gene ID (NCBI) 10856
RRIDAB_2918610
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y230
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)