验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, SGC-7901 cells, HeLa cells, BGC-823 cells, mouse brain tissue, rat brain tissue |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83601-2-RR targets RUFY3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35558 Product name: Recombinant human RUFY3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 110-469 aa of BC051716 Sequence: HCLKHGLKAKKTFLGQNKSFWGPLELVEKLVPEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEPNALMMEEEGAIIAGLLVGLNVIDANFCMKGEDLDSQVGVIDFSMYLKDGNSSKGTEGDGQITAILDQKNYVEELNRHLNATVNNLQAKVDALEKSNTKLTEELAVANNRIITLQEEMERVKEESSYILESNRKGPKQDRTAEGQALSEARKHLKEETQLRLDVEKELEMQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVKQKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RUN and FYVE domain containing 3 |
| 别名 | 240519E7, KIAA0871, Protein RUFY3, Rap2 interacting protein x, Rap2-interacting protein x |
| 计算分子量 | 53 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC051716 |
| 基因名称 | RUFY3 |
| Gene ID (NCBI) | 22902 |
| RRID | AB_3671215 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7L099 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RUFY3 (RUN and FYVE domain-containing protein 3) was originally described as lacking part of the C-terminal CC2 and FYVE domains. The interaction of RUFY3 with the filamentous actin network through a complex formed together with Rap2 and Fascin 1 (FSCN1) is critical for axonogenesis and growth cone development (PMID: 37463962). RUFY3 also seems to play a crucial role in promoting cancer development (PMID: 34510031).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for RUFY3 antibody 83601-2-RR | Download protocol |
| WB protocol for RUFY3 antibody 83601-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
