验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10740 targets RTN4/NOGO in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1164 Product name: Recombinant human RTN4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC007109 Sequence: MDGQKKNWKDKVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNCTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSVPVIYERHQAQIDHYLGLANKNVKDAMAKIQAKIPGLKRKAE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | reticulon 4 |
| 别名 | ASY, Foocen, KIAA0886, Nbla00271, Nbla10545, Neurite outgrowth inhibitor, NI220/250, NOGO, NOGO A, Nogo B, Nogo C, Nogo protein, NOGOA, NOGOC, NSP, NSP CL, reticulon 4, Reticulon 5, RTN X, RTN4, RTN4 A, RTN4 B1, RTN4 B2, RTN4 C, SP1507 |
| 计算分子量 | 130 kDa |
| 观测分子量 | 190-210 kDa, 45 kDa, 22-25 kDa |
| GenBank蛋白编号 | BC007109 |
| 基因名称 | NOGO |
| Gene ID (NCBI) | 57142 |
| RRID | AB_3672462 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQC3 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RTN4/NOGO antibody CL488-10740 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
