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验证数据展示

  • WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

    WB analysis using 12055-2-AP (same clone as 12055-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12055-2-AP (RTN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12055-2-AP (same clone as 12055-2-PBS)

    IP experiment of HeLa using 12055-2-AP (same clone as 12055-2-PBS)

    IP result of anti-RTN3 (IP:12055-2-AP, 4ug; Detection:12055-2-AP 1:400) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 12055-2-AP (same clone as 12055-2-PBS)

    IHC staining of rat brain using 12055-2-AP (same clone as 12055-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12055-2-AP (RTN3 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 12055-2-AP (same clone as 12055-2-PBS)

    IHC staining of rat brain using 12055-2-AP (same clone as 12055-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12055-2-AP (RTN3 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12055-2-AP (same clone as 12055-2-PBS)

    IF Staining of mouse brain using 12055-2-AP (same clone as 12055-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using RTN3 antibody (12055-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12055-2-PBS in a different storage buffer formulation.

RTN3 Polyclonal antibody, PBS Only

RTN3 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 12055-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

ASYIP, HAP, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12055-2-PBS targets RTN3 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2685

Product name: Recombinant human RTN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-236 aa of BC011394

Sequence: MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAVHDLIFWRDVKKTGFVFGTTLIMLLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMVHINRALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVGIARDQTKSIVEKIQAKLPGIAKKKAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 reticulon 3
别名 ASYIP, HAP, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II
计算分子量 113 kDa
观测分子量 24 kDa, 100-113 kDa
GenBank蛋白编号BC011394
基因名称 RTN3
Gene ID (NCBI) 10313
RRIDAB_2301357
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95197
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.

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