RTN3 Polyclonal antibody
RTN3 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, human brain tissue, HeLa cells, U-87 MG cells, U2OS cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12055-2-AP targets RTN3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, monkey |
Immunogen | RTN3 fusion protein Ag2685 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | reticulon 3 |
Synonyms | ASYIP, HAP, NSP like protein 2, NSP like protein II, NSPL2, NSPLII, reticulon 3, RTN3, RTN3 A1 |
Calculated Molecular Weight | 113 kDa |
Observed Molecular Weight | 24 kDa, 100-113 kDa |
GenBank Accession Number | BC011394 |
Gene Symbol | RTN3 |
Gene ID (NCBI) | 10313 |
RRID | AB_2301357 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95197 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RTN3 antibody 12055-2-AP | Download protocol |
IHC protocol for RTN3 antibody 12055-2-AP | Download protocol |
IP protocol for RTN3 antibody 12055-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
Acta Neuropathol Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS | ||
J Pineal Res Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression | ||
Dev Cell TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules. | ||
Acta Neuropathol Commun Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia. |