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验证数据展示

  • WB analysis using 27667-1-AP (same clone as 27667-1-PBS)

    WB analysis using 27667-1-AP (same clone as 27667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27667-1-AP (RSL1D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 27667-1-AP (same clone as 27667-1-PBS)

    IHC staining of mouse colon using 27667-1-AP (same clone as 27667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27667-1-AP (RSL1D1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 27667-1-AP (same clone as 27667-1-PBS)

    IHC staining of mouse colon using 27667-1-AP (same clone as 27667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27667-1-AP (RSL1D1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27667-1-PBS in a different storage buffer formulation.

RSL1D1 Polyclonal antibody, PBS Only

RSL1D1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27667-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27667-1-PBS targets RSL1D1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24923

Product name: Recombinant human RSL1D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 269-490 aa of BC112228

Sequence: SNWDEATKRSLLNKKKKEARRKRRERNFEKQKERKKKRQQARKTASVLSKDDVAPESGDTTVKKPESKKEQTPEHGKKKRGRGKAQVKATNESEDEIPQLVPIGKKTPANEKVEIQKHATGKKSPAKSPNPSTPRGKKRKALPASETPKAAESETPGKSPEKKPKIKEEAVKEKSPSLGKKDARQTPKKPEAKFFTTPSKSVRKASHTPKKWPKKPKVPQST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal L1 domain containing 1
别名
计算分子量 490 aa, 55 kDa
观测分子量55-70 kDa
GenBank蛋白编号BC112228
基因名称 RSL1D1
Gene ID (NCBI) 26156
RRIDAB_3085982
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76021
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RL1D1, Ribosomal L1 domain-containing protein 1, also named CSIG, a member of the universal ribosomal protein uL1 family. RL1D1 regulates cellular senescence through inhibition of PTEN translation. It acts as a pro-apoptotic regulator in response to DNA damage (PMID: 18678645, PMID: 22419112). RSL1D1 promoted the progression of colorectal cancer through RAN-mediated autophagy suppression. RSL1D1 can be detected 70 kDa (PMID:35013134).

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