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验证数据展示

  • WB analysis using 27667-1-AP

    WB analysis using 27667-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27667-1-AP (RSL1D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 27667-1-AP

    IHC staining of mouse colon using 27667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27667-1-AP (RSL1D1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27667-1-AP

    IHC staining of mouse colon using 27667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27667-1-AP (RSL1D1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RSL1D1 Polyclonal antibody

RSL1D1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27667-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

CATX 11, CATX11, CSIG, L12, PBK1, Protein PBK1, RSL1D1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-251 cells, HCT 116 cells, HEK-293 cells, SW480 cells, K-562 cells, HepG2 cells, PC-3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27667-1-AP targets RSL1D1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 RSL1D1 fusion protein Ag24923 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal L1 domain containing 1
别名 CATX 11, CATX11, CSIG, L12, PBK1, Protein PBK1, RSL1D1
计算分子量 490 aa, 55 kDa
观测分子量55-70 kDa
GenBank蛋白编号BC112228
基因名称 RSL1D1
Gene ID (NCBI) 26156
RRIDAB_3085982
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76021
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RL1D1, Ribosomal L1 domain-containing protein 1, also named CSIG, a member of the universal ribosomal protein uL1 family. RL1D1 regulates cellular senescence through inhibition of PTEN translation. It acts as a pro-apoptotic regulator in response to DNA damage (PMID: 18678645, PMID: 22419112). RSL1D1 promoted the progression of colorectal cancer through RAN-mediated autophagy suppression. RSL1D1 can be detected 70 kDa (PMID:35013134).

实验方案

Product Specific Protocols
WB protocol for RSL1D1 antibody 27667-1-APDownload protocol
IHC protocol for RSL1D1 antibody 27667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols