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验证数据展示

  • WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    WB analysis of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • WB analysis using 23762-1-AP (same clone as 23762-1-PBS)

    WB analysis using 23762-1-AP (same clone as 23762-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    IP experiment of K-562 using 23762-1-AP (same clone as 23762-1-PBS)

    IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

    IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

    IHC staining of human kidney using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 23762-1-AP (same clone as 23762-1-PBS)

    IHC staining of mouse colon using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 23762-1-AP (same clone as 23762-1-PBS)

    IHC staining of mouse kidney using 23762-1-AP (same clone as 23762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23762-1-AP (RSK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 23762-1-AP (same clone as 23762-1-PBS)

    IF Staining of mouse skeletal muscle using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using RSK2 antibody (23762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

    IF Staining of HepG2 using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23762-1-AP (RSK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

    IF Staining of mouse brain using 23762-1-AP (same clone as 23762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23762-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RSK2 Polyclonal antibody, PBS Only

RSK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 23762-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

RPS6KA3, CLS, EC:2.7.11.1, HU 3, Insulin-stimulated protein kinase 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23762-1-PBS targets RSK2 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20620

Product name: Recombinant human RPS6KA3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 666-740 aa of BC096301

Sequence: QRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 3
别名 RPS6KA3, CLS, EC:2.7.11.1, HU 3, Insulin-stimulated protein kinase 1
计算分子量 740 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC096301
基因名称 RSK2
Gene ID (NCBI) 6197
RRIDAB_2879318
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51812
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

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