- Featured Product
- KD/KO Validated
RSK2 Polyclonal antibody
RSK2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, K-562 cells, MCF-7 cells, HepG2 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 7 publications below |
产品信息
The immunogen of 23762-1-AP is RSK2 Fusion Protein expressed in E. coli.
Tested Applications | IHC, IP, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | RSK2 fusion protein Ag20620 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 3 |
Synonyms | CLS, HU 3, ISPK 1, ISPK1, MAPKAP kinase 1b, MAPKAPK 1b, MAPKAPK1B, MRX19, p90 RSK 3, p90 RSK2, p90RSK3, pp90RSK2, Ribosomal S6 kinase 2, RPS6KA3, RSK, RSK 2, RSK2, S6K alpha 3, S6K alpha3 |
Calculated Molecular Weight | 740 aa, 84 kDa |
Observed Molecular Weight | 84 kDa |
GenBank Accession Number | BC096301 |
Gene Symbol | RPS6KA3 |
Gene ID (NCBI) | 6197 |
RRID | AB_2879318 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P51812 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RSK2 antibody 23762-1-AP | Download protocol |
IHC protocol for RSK2 antibody 23762-1-AP | Download protocol |
IF protocol for RSK2 antibody 23762-1-AP | Download protocol |
IP protocol for RSK2 antibody 23762-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer. | ||
Cell Death Differ β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer. | ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. | ||
Int J Biochem Cell Biol DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo. | ||
Prostate MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components. | ||
PLoS One A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction |