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验证数据展示

  • WB analysis using 24734-1-AP

    WB analysis using 24734-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24734-1-AP (RSBN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24734-1-AP

    IF Staining of A431 using 24734-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RSBN1 antibody (24734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 24734-1-AP

    IF Staining of HeLa using 24734-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RSBN1 antibody (24734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RSBN1 Polyclonal antibody

RSBN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24734-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ROSBIN, Rsbn, RSBN 1, Rsbp

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, PC-3 cells
Positive IF/ICC detected inHeLa cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24734-1-AP targets RSBN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20895

Product name: Recombinant human RSBN1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-411 aa of BC026155

Sequence: MAAQVGAVRVVRAVAAQEEPDKEGKEKPHAGVSPRGVKRQRRSSSGGSQEKRGRPSQEPPLAPPHRRRRSRQHPGPLPPTNAAPTVPGPVEPLLLPPPPPPSLAPAGPAVAAPLPAPSTSALFTFSPLTVSAAGPKHKGHKERHKHHHHRGPDGDPSSCGTDLKHKDKQENGERTGGVPLIKAPKRETPDENGKTQRADDFVLKKIKKKKKKKHREDMRGRRLKMYNKEVQTVCAGLTRISKEILTQGQINSTSGLNKESFRYLKDEQLCRLNLGMQEYRVPQGVQTPFMTHQEHSIRRNFLKTGTKFSNFIHEEHQSNGGALVLHAYMDELSFLSPMEMERFSEEFLALTFSENEKNAAYYALAIVHGAAAYLPDFLDYFAFNFPNTPVKMEILGKKDIETTTISNFHTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 round spermatid basic protein 1
别名 ROSBIN, Rsbn, RSBN 1, Rsbp
计算分子量 411 aa, 46 kDa
观测分子量90 kDa
GenBank蛋白编号BC026155
基因名称 RSBN1
Gene ID (NCBI) 54665
RRIDAB_3742124
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VWQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RSBN1 is expressed not only in the testes, but also in the brain and ovaries. RSBN1 could play a role in both meiotic and post-meiotic progression, suggesting that it may also be involved in oogenesis. On the other hand, RSBN1 may be involved in brain development and function, as well as the regulation of neuronal cells, since another H4K20 demethylase, PHF8, regulates neuronal cell survival and brain development, while LSD1 regulates memory formation (PMID: 20622853, 26214369, 34185806).

实验方案

Product Specific Protocols
IF protocol for RSBN1 antibody 24734-1-APDownload protocol
WB protocol for RSBN1 antibody 24734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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