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  • Featured Product
  • KD/KO Validated

RRM1 Monoclonal antibody, PBS Only

RRM1 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5H6F3

Cat No : 60073-2-PBS

Print datasheet

Synonyms

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1

验证数据展示

  • WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    WB analysis of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60073-2-Ig (RRM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRM1 antibody included
  • IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    IHC staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60073-2-Ig (RRM1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RRM1 antibody included
  • IP experiment of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    IP experiment of K-562 using 60073-2-Ig (same clone as 60073-2-PBS)

    IP result of anti-RRM1 (IP:60073-2-Ig, 5ug; Detection:60073-2-Ig 1:1000) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60073-2-Ig (same clone as 60073-2-PBS)

    IF Staining of HepG2 using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    IF Staining of human breast cancer using 60073-2-Ig (same clone as 60073-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RRM1 antibody (60073-2-Ig, Clone: 5H6F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60073-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RRM1 Ready-To-Use IHC Kit. RRM1 primary antibody included.

产品信息

60073-2-PBS targets RRM1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF
Tested Reactivity human
Immunogen RRM1 fusion protein Ag0789 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribonucleotide reductase M1
Synonyms R1, ribonucleotide reductase M1, RIR1, RR1, RRM1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23921
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).