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CoraLite® Plus 647-conjugated RRBP1 Polyclonal antibody

RRBP1 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-22015

Print datasheet

Synonyms

DKFZp586A1420, ES/130, ES/130 related protein, ES130, FLJ36146, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp

验证数据展示

  • FC experiment of HeLa using CL647-22015

    FC experiment of HeLa using CL647-22015

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human RRBP1 (CL647-22015) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL647-22015

    IF Staining of HepG2 using CL647-22015

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 RRBP1 antibody (CL647-22015) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22015-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-22015 targets RRBP1 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse
Immunogen RRBP1 fusion protein Ag16648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosome binding protein 1 homolog 180kDa (dog)
Synonyms DKFZp586A1420, ES/130, ES/130 related protein, ES130, FLJ36146, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp
Calculated Molecular Weight 1410 aa, 152 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC128577
Gene Symbol RRBP1
Gene ID (NCBI) 6238
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2E9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 RRBP1 antibody CL647-22015Download protocol
FC protocol for CL Plus 647 RRBP1 antibody CL647-22015Download protocol
Standard Protocols
Click here to view our Standard Protocols