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验证数据展示

  • IF Staining of HeLa using 82890-1-RR

    IF Staining of HeLa using 82890-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • IF Staining of NIH/3T3 using 82890-1-RR

    IF Staining of NIH/3T3 using 82890-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • IF Staining of NIH/3T3 using 82890-1-RR

    IF Staining of NIH/3T3 using 82890-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RRBP1 antibody (82890-1-RR, Clone: 230159G6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • FC experiment of U-2 OS using 82890-1-RR

    FC experiment of U-2 OS using 82890-1-RR

    (blue)1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human RRBP1 (82890-1-RR, Clone:230159G6) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of HeLa using 82890-1-RR

    FC experiment of HeLa using 82890-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-1-RR, Clone:230159G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82890-1-RR

    Affinity and Kinetic Characterization of 82890-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82890-1-RR against Human RRBP1 were performed. The affinity constant is below 1 pM.

RRBP1 Recombinant monoclonal antibody

RRBP1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82890-1-RR

产品说明书

CloneNo. 230159G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra), ELISA

别名

180 kDa ribosome receptor homolog, 230159G6, ES/130 related protein, ES/130-related protein, Ribosome-binding protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells, HeLa cells
Positive FC (Intra) detected inU-2 OS, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RRBP1 Ready-To-Use IHC Kit. RRBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82890-1-RR targets RRBP1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16648

Product name: Recombinant human RRBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1061-1410 aa of BC128577

Sequence: AQTMEALLALLPELSVLAQQNYTEWLQDLKEKGPTLLKHPPAPAEPSSDLASKLREAEETQSTLQAECDQYRSILAETEGMLRDLQKSVEEEEQVWRAKVGAAEEELQKSRVTVKHLEEIVEKLKGELESSDQVREHTLHLEAELEKHMAAASAECQNYAKEVAGLRQLLLESQSQLDAAKSEAQKQSDELALVRQQLSEMKSHVEDGDIAGAPASSPEAPPAEQDPVQLKTQLEWTEAILEDEQTQRQKLTAEFEEAQTSACRLQEELEKLRTAGPLESSETEEASQLKERLEKEKKLTSDLGRAATRLQELLKTTQEQLAREKDTVKKLQEQLEKAEDGSSSKEGTSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosome binding protein 1 homolog 180kDa (dog)
别名 180 kDa ribosome receptor homolog, 230159G6, ES/130 related protein, ES/130-related protein, Ribosome-binding protein 1
计算分子量 1410 aa, 152 kDa
观测分子量108 kDa, 152 kDa
GenBank蛋白编号BC128577
基因名称 RRBP1
Gene ID (NCBI) 6238
RRIDAB_3670614
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2E9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

实验方案

Product Specific Protocols
IF protocol for RRBP1 antibody 82890-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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