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验证数据展示

  • WB analysis of DU 145 using 17296-1-AP (same clone as 17296-1-PBS)

    WB analysis of DU 145 using 17296-1-AP (same clone as 17296-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 17296-1-AP (RPS4Y1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 17296-1-AP (same clone as 17296-1-PBS)

    IHC staining of human appendicitis using 17296-1-AP (same clone as 17296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17296-1-AP (RPS4Y1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 17296-1-AP (same clone as 17296-1-PBS)

    IF Staining of HepG2 using 17296-1-AP (same clone as 17296-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS4Y1 antibody (17296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17296-1-PBS in a different storage buffer formulation.

RPS4Y1 Polyclonal antibody, PBS Only

RPS4Y1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17296-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17296-1-PBS targets RPS4Y1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11176

Product name: Recombinant human RPS4Y1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-263 aa of BC010286

Sequence: KKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIVFLRNRLKYALTGDEVKKICMQRFIKIDGKVRVDVTYPAGFMDVISIEKTGEHFRLVYDTKGRFAVHRITVEEAKYKLCKVRKITVGVKGIPHLVTHDARTIRYPDPVIKVNDTVQIDLGTGKIINFIKFDTGNLCMVIGGANLGRVGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGNGNKPWISLPRGKGIRLTVAEERDKRLATKQSSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S4, Y-linked 1
别名 Small ribosomal subunit protein eS4, Y isoform 1, RPS4Y, PRO2646, 40S ribosomal protein S4
计算分子量 263 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC010286
基因名称 RPS4Y1
Gene ID (NCBI) 6192
RRIDAB_2180889
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22090
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS4Y1, a Y-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). RPS4Y maps to a 90-kb segment that has been implicated in Turner syndrome. As a homolog with RPS4X, the antibody can recognize endogenous level of RPS4Y and RPS4X, at 29-32kDa.

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