×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of mouse cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

    WB analysis of mouse cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14946-1-AP (RPS27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

  • IHC staining of human cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

    IHC staining of human cerebellum using 14946-1-AP (same clone as 14946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 14946-1-AP (same clone as 14946-1-PBS)

    IHC staining of human endometrial cancer using 14946-1-AP (same clone as 14946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14946-1-AP (RPS27A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14946-1-AP (same clone as 14946-1-PBS)

    IF Staining of HeLa using 14946-1-AP (same clone as 14946-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS27A antibody (14946-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 14946-1-AP (same clone as 14946-1-PBS)

    FC experiment of HeLa using 14946-1-AP (same clone as 14946-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RPS27A (14946-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14946-1-PBS in a different storage buffer formulation.

RPS27A Polyclonal antibody, PBS Only

RPS27A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 14946-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CEP80, HUBCEP80, ribosomal protein S27a, Small ribosomal subunit protein eS31, UBA80

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

14946-1-PBS targets RPS27A in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6750

Product name: Recombinant human RPS27A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-156 aa of BC001392

Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S27a
别名 CEP80, HUBCEP80, ribosomal protein S27a, Small ribosomal subunit protein eS31, UBA80
计算分子量18 kDa
观测分子量 9 kDa, 18 kDa
GenBank蛋白编号BC001392
基因名称 RPS27A
Gene ID (NCBI) 6233
RRIDAB_2878093
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62979
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]

||
New chat

Able™

正在加载，请稍候...