RPS27 Polyclonal antibody
RPS27 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, SGC-7901 cells, Jurkat cells |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
15355-1-AP targets RPS27 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IF/ICC, IHC, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | RPS27 fusion protein Ag7601 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribosomal protein S27 |
| Synonyms | 40S ribosomal protein S27, Metallopan stimulin 1, MPS 1, MPS1, ribosomal protein S27, RPS27 |
| Calculated Molecular Weight | 9 kDa |
| Observed Molecular Weight | 9 kDa |
| GenBank Accession Number | BC002658 |
| Gene Symbol | RPS27 |
| Gene ID (NCBI) | 6232 |
| RRID | AB_2180509 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42677 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IHC protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IF protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IP protocol for RPS27 antibody 15355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. | ||
FEBS Lett The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells. | ||
Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation | ||
