验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, SGC-7901 cells, Jurkat cells |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
15355-1-AP targets RPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7601 Product name: Recombinant human RPS27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC002658 Sequence: MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S27 |
| 别名 | MPS-1, MPS1, MPS 1, Metallopan-stimulin 1, Metallopan stimulin 1 |
| 计算分子量 | 9 kDa |
| 观测分子量 | 9 kDa |
| GenBank蛋白编号 | BC002658 |
| 基因名称 | RPS27 |
| Gene ID (NCBI) | 6232 |
| RRID | AB_2180509 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42677 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IHC protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IF protocol for RPS27 antibody 15355-1-AP | Download protocol |
| IP protocol for RPS27 antibody 15355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. | ||
FEBS Lett The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells. | ||
Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation | ||
J Mol Biol Defective Human SRP Induces Protein Quality Control and Triggers Stress Response |
