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RPS25 Polyclonal antibody

RPS25 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23599-1-AP

Print datasheet

Synonyms

40S ribosomal protein S25, ribosomal protein S25, RPS25

验证数据展示

  • WB analysis using 23599-1-AP

    WB analysis using 23599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23599-1-AP (RPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 23599-1-AP

    IHC staining of human breast cancer using 23599-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23599-1-AP (RPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 23599-1-AP

    IHC staining of human liver using 23599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23599-1-AP (RPS25 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 23599-1-AP

    IF Staining of MCF-7 using 23599-1-AP

    Immunofluorescent analysis of MCF-7 cells using 23599-1-AP (RPS25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 23599-1-AP is RPS25 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RPS25 fusion protein Ag7890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S25
Synonyms 40S ribosomal protein S25, ribosomal protein S25, RPS25
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 15-17 kDa
GenBank Accession NumberBC004986
Gene Symbol RPS25
Gene ID (NCBI) 6230
RRIDAB_11183757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62851
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting.

实验方案

Product Specific Protocols
WB protocol for RPS25 antibody 23599-1-APDownload protocol
IHC protocol for RPS25 antibody 23599-1-APDownload protocol
IF protocol for RPS25 antibody 23599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Nano

Platelet-Derived Apoptotic Vesicles Promote Bone Regeneration via Golgi Phosphoprotein 2 (GOLPH2)-AKT Signaling Axis

Authors - Yuhe Jiang
View Article
humanWB

Mol Cell Proteomics

Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma.

Authors - Chiung-Hung Hsu
View Article