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验证数据展示

  • WB analysis using 29834-1-AP (same clone as 29834-1-PBS)

    WB analysis using 29834-1-AP (same clone as 29834-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29834-1-AP (RPS23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29834-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 29834-1-AP (same clone as 29834-1-PBS)

    IF Staining of MCF-7 using 29834-1-AP (same clone as 29834-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS23 antibody (29834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29834-1-PBS in a different storage buffer formulation.

RPS23 Polyclonal antibody, PBS Only

RPS23 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29834-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Small ribosomal subunit protein uS12

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® RPS23 Ready-To-Use IHC Kit. RPS23 primary antibody included.

产品信息

29834-1-PBS targets RPS23 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31190

Product name: Recombinant human RPS23 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-143 aa of NM_001025

Sequence: GKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S23
别名 Small ribosomal subunit protein uS12
计算分子量 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号NM_001025
基因名称 RPS23
Gene ID (NCBI) 6228
RRIDAB_3669698
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62266
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS23 (40S ribosomal protein S23), also called small ribosomal subunit protein uS12, is a subunit of the 40S ribosome and the first precursor of the small eukaryotic ribosomal subunit (PMID: 34516797). It is positioned in the decoding center of the ribosome that serves to maintain translational fidelity by monitoring the complementarity between the mRNA codons being translated and the anti-codons of aminoacyl-tRNAs (PMID: 28257692; 23636399).

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