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Species Reactivity
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验证数据展示

  • WB analysis using 29834-1-AP

    WB analysis using 29834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29834-1-AP (RPS23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 29834-1-AP

    IF Staining of MCF-7 using 29834-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RPS23 antibody (29834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RPS23 Polyclonal antibody

RPS23 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29834-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells, human placenta tissue, mouse ovary tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29834-1-AP targets RPS23 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RPS23 fusion protein Ag31190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S23
别名
计算分子量 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号NM_001025
基因名称 RPS23
Gene ID (NCBI) 6228
RRIDAB_3669698
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62266
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS23 (40S ribosomal protein S23), also called small ribosomal subunit protein uS12, is a subunit of the 40S ribosome and the first precursor of the small eukaryotic ribosomal subunit (PMID: 34516797). It is positioned in the decoding center of the ribosome that serves to maintain translational fidelity by monitoring the complementarity between the mRNA codons being translated and the anti-codons of aminoacyl-tRNAs (PMID: 28257692; 23636399).

实验方案

Product Specific Protocols
WB protocol for RPS23 antibody 29834-1-APDownload protocol
IF protocol for RPS23 antibody 29834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols