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验证数据展示

  • IF Staining of HeLa using CL488-68270

    IF Staining of HeLa using CL488-68270

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RPS20 antibody (CL488-68270, Clone: 3E9B4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-68270

    FC experiment of HeLa using CL488-68270

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RPS20 (CL488-68270, Clone:3E9B4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated RPS20 Monoclonal antibody

RPS20 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68270

产品说明书

CloneNo. 3E9B4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS20 Ready-To-Use IHC Kit. RPS20 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68270-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68270 targets RPS20 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 RPS20 fusion protein Ag8275 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S20
别名 Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4
计算分子量 13 kDa
观测分子量16 kDa
GenBank蛋白编号BC007507
基因名称 RPS20
Gene ID (NCBI) 6224
RRIDAB_3084463
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60866
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPS20 antibody CL488-68270Download protocol
FC protocol for CL Plus 488 RPS20 antibody CL488-68270Download protocol
Standard Protocols
Click here to view our Standard Protocols